Date: 01.09.2009, Time: 22.00
Relax life
Do we have in the city a healthy and quality life, do we have calmness, and secure live in the city? Saalad has non of these. There are so much problems in the city which destroys our nerves and make us to become a bad person, because nothing is healthy. Air,water, soil, foods are all polluted.
How about you? Do you have a relax life in the city?
There are some people who have a good live in a village which the below pictures speak for themselves there are some family who have built a suitable shelter in a remote area and have quiet life.

House Dinning Room Garden
Map building a house Bedroom

Relaxed family

Seating Room

Stairs
Date:02.09.09. Time: 22.00
AVANTAGES:
- No council tax, road tax, income tax, heritage tax, T.V license, no VAT, no road tax, no insurance, no bank charges.
- No noise pollution, no disturbance, no troubles, no crime.
- No parking problem, No penalty ticket, no traffic, no suffering from air pollution, no accident.
- No construction and planing problem, no building materials problem, no decoration problems
- Good air, good water, good foods, good environment, good feelings, good health, good time to do things which one wish to do.
- Children are secure, education is good, because there is no T.V no junk foods, no game, no bad words, no leis and news fabrication, children will not see no violence on T.V
- Family have more time to spent together, family have more time to learn from each other.
DISADVANTAGES:
- After a year life become boring because of routine style life.
- Isolation will be a long term problems.
- Lack of knowledge about the advancement and civilization of the real world.
- Learning limitation.
- Hard work to do everything themselves.
- There will no help in an emergency case.
- In case of health problem, there is no hospital for prompt medication.
Date: 13.09.2009. Time: 22.00
GENGIZ KHAN MONGOL

Goghtay Gengiz Khan
He came to power by uniting many of the nomadic tribes of northeast Asia. After founding the Mongol Empire and being proclaimed "Genghis Khan", he started the Mongol invasions and raids of the Kara-Khitan Khanate, Caucasus, Khwarezmid Empire, Western Xia and Jin dynasties. During his life, the Mongol Empire eventually occupied a substantial portion of Central Asia.
Before Genghis Khan died, he assigned Ogedei Khan as his successor and split his empire into khanates among his sons and grandsons. He died in 1227 after defeating the Tanguts. He was buried in an unmarked grave somewhere in Mongolia at a location unknown. His descendants went on to stretch the Mongol Empire across most of Eurasia by conquering and/or creating vassal states out of all of modern-day China, Korea, the Caucasus, Central Asian countries, and substantial portions of modern Eastern Europe and the Middle East.
Family Tree
Temüjin was related on his father's side to Khabul Khan, Ambaghai and Qutula Khan who had headed the Mongol confederation. When the Jin Dynasty switched support from the Mongols to the Tatars in 1161, they destroyed Qabul Khan.[4] Genghis' father, Yesugei (leader of the Borjigin and nephew to Ambaghai and Qutula Khan), emerged as the head of the ruling clan of the Mongols, but this position was contested by the rival Tayichi’ud clan, who descended directly from Ambaghai. When the Tatars grew too powerful after 1161, the Jin switched their support from the Tatars to the Keraits.
Birth
Because of the lack of contemporary written records, there is very little factual information about the early life of Temüjin. The few sources that provide insight into this period are often conflicting.
Temüjin was born 1162[2] in a Mongol tribe near Burkhan Khaldun mountain and the Onon and Kherlen Rivers in modern day Mongolia, not far from its current capital Ulaanbaatar. The Secret History of the Mongols reports that Temüjin was born with a blood clot grasped in his fist, a sign that he was destined to become a great leader. He was the third-oldest son of his father Yesükhei, a minor tribal chief of the Kiyad and an ally of Ong Khan of the Kerait tribe,[5] and the oldest son of his mother Hoelun. According to the Secret History, Temüjin was named after a Tatar chieftain that his father had just captured. The name also suggests that they may have been descended from a family of blacksmiths (see section Name and title below).
No accurate portraits of Genghis exist today, and any surviving depictions are considered to be artistic interpretations. Persian historian Rashid al-Din recorded in his "Chronicles" that the legendary "glittering" ancestor of Genghis was tall, long-bearded, red-haired, and green-eyed. Rashid al-Din also described the first meeting of Genghis and Kublai Khan, when Genghis was shocked to find that Kublai had not inherited his red hair.[8] Also according to al-Din Genghis's Borjigid clan, had a legend involving their origins: it began as the result of an affair between Alan-ko and a stranger to her land, a glittering man who happened to have red hair and bluish-green eyes. Modern historian Paul Ratchnevsky has suggested in his Genghis biography that the "glittering man" may have been from the Kyrgyz people, who historically displayed these same characteristics. Controversies aside, the closest depiction generally accepted by most historians is the portrait currently in the National Palace Museum in Taipei, Taiwan (see picture above).
Early life and family
Temüjin had three brothers named Khasar (or Qasar), Khajiun, and Temüge, and one sister named Temülen (or Temülin), as well as two half-brothers named Bekhter and Belgutei. Like many of the nomads of Mongolia, Temüjin's early life was difficult. At nine years old, as part of the marriage arrangement, he was delivered by his father to the family of his future wife Börte, who was a member of the same tribe as his mother. He was to live there in service to Sansar, the head of the household, until he reached the marriageable age of 12. While heading home, his father was poisoned during a meal with the neighbouring Tatars, who had long been enemies of the Mongols. Temüjin returned home to claim the position of khan. However, his father's tribe refused to be led by a boy so young. They abandoned Hoelun and her children, leaving them without protection.
For the next several years, Hoelun and her children lived in poverty, surviving primarily on wild fruits, marmots, and other small game hunted by Temüjin and his brothers. It was during one hunting excursion that 13-year-old Temüjin killed his half-brother, Bekhter, during a fight which resulted from a dispute over hunting spoils.[9] This incident cemented his position as head of the household.
At this time, none of the tribal confederations of Mongolia were united politically, and arranged marriages were often used to solidify temporary alliances. Temujin grew up observing the tough political climate of Mongolia, surrounded by tribal warfare, thievery, raids, corruption and continuing acts of revenge carried out between the various confederations, all compounded by interference from foreign forces such as the Chinese dynasties to the south. Temüjin's mother Ho'elun taught him many lessons about the unstable political climate of Mongolia, especially the need for alliances.
As previously arranged by his father, Temüjin married Börte of the Olkut'hun tribe when he was around 16 in order to cement alliances between their respective tribes. Börte had four sons, Jochi (1185–1226), Chagatai (1187—1241), Ögedei (1189—1241), and Tolui (1190–1232). Genghis Khan also had many other children with his other wives, but they were excluded from the succession, and records of daughters are nonexistent. Soon after Börte's marriage to Temüjin, she was kidnapped by the Merkits, and reportedly given away as a wife. Temüjin rescued her with the help of his friend and future rival, Jamuka, and his protector, Ong Khan of the Kerait tribe. She gave birth to a son, Jochi, nine months later, clouding the issue of his parentage. Despite speculation over Jochi, Börte would be his only empress, though Temüjin did follow tradition by taking several morganatic wives.[10]
Religion
Genghis Khan's religion is widely speculated to be Shamanism or Tengriism, which was very likely among nomadic Mongol-Turkic tribes of Central Asia. But he was very tolerant religiously, and interested to learn philosophical and moral lessons from other religions. To do so, he consulted among others with Christian missionaries, Muslim merchants, and the Taoist monk Qiu Chuji.
YASSA GENGHIS KHAN
2. Leaders of a religion, preachers, monks, persons who are dedicated to religious practice, the criers of mosques, physicians and those who bathe the bodies of the dead are to be freed from public charges.
3. It is forbidden under penalty of death that any one, whoever he is, shall be proclaimed emperor unless he has been elected previously by the princes, khans, officers, and other Mongol nobles in a general council.
4. It is forbidden chieftains of nations and clans subject to the Mongols to hold honorary tiles.
5. Forbidden to ever make peace with a monarch, a prince or a people who have not submitted.
6.The ruling that divides men of the army into tens, hundreds, thousands, and ten thousands is to be maintained. This arrangement serves to raise an army in a short time, and to form the units of commands.
7. The moment a campaign begins, each soldier must receive his arms from the hand of the officer who has them in charge. The soldier must keep them in good order, and have them inspected by his officer before a battle.
8. Forbidden, under death penalty, to pillage the enemy before the general commanding gives permission; but after this permission is given the soldier must have the same opportunity as the officer, and must be allowed to keep what he has carried off, provided he has paid his share to the receiver for the emperor.
9. To keep the men of the army exercised, a great hunt shall be held every winter. On this account, it is forbidden any man of the empire to kill from the month of March to October, deer, bucks, roe-bucks, hares, wild ass and some birds.
10. Forbidden, to cut the throats of animals slain for food; they must be bound, the chest opened and the heart pulled out by the hand of the hunter.
11. It is permitted to eat the blood and entrails of animals-though this was forbidden before now.
12. (A list of privileges and immunities assured the chieftains and officers of the new empire.)
13. Every man who does not go to war must work for the empire, without reward, for a certain time.
14. Men guilty of the theft of a horse or steer or a thing of equal value will be punished by death and their bodies cut into two parts. For lesser thefts the punishment shall be, according to the value of the thing stolen, a number of blows of a staff-seven, seventeen, twenty-seven, up to seven hundred. But this bodily punishment may be avoided by paying nine times the worth of the thing stolen.
15.No subject of the empire may take a Mongol for servant or slave. Every man, except in rare cases, must join the army.
16. To prevent the flight of alien slaves, it is forbidden to give them asylum, food or clothing, under pain of death. Any man who meets an escaped slave and does not bring him back to his master will be punished in the same manner.
17. The law of marriage orders that every man shall purchase his wife, and that marriage between the first and second degrees of kinship is forbidden. A man may marry two sisters, or have several concubines. The women should attend to the care of property, buying and selling at their pleasure. Men should occupy themselves only with hunting and war. Children born of slaves are legitimate as the children of wives. The offspring of the first woman shall be honored above other children and shall inherit everything.
18. Adultery is to be punished by death, and those guilty of it may be slain out of hand.
19. If two families wish to be united by marriage and have only young children, the marriage of these children is allowed, if one be a boy and the other a girl. If the children are dead, the marriage contract may still be drawn up.
20. It is forbidden to bathe or wash garments in running water during thunder.
21. Spies, false witnesses, all men given to infamous vices, and sorcerers are condemned to death.
22. Officers and chieftains who fail in their duty, or do not come at the summons of the Khan are to be slain, especially in remote districts. If their offense be less grave, they must come in person before the Khan."
TIMUR LANG

A descendant of Mongol conquerors, Timur - whose tribe had become Turkicized in identity and language[6] and Persianized in culture and religion - aspired to recreate the empire of his ancestors. He was a military genius who loved to play chess in his spare time to improve his military tactics and skill. And although he wielded absolute power, he never called himself more than an emir.
His full name in the Arabic tradition of ism, nasba, and nisbat was Tīmūr bin Taraġay Barlas. Temür means "iron" in the Turkish language and in Mongolian (compare Temüjin "ironworker", the given name of Genghis Khan). The term temür is ultimately derived from a Buddhist Hybrid Sanskrit word (čimara ("iron").
After his marriage into Genghis Khan's family, he took the name Timūr Gurkānī Gurkān being the Persianized form of the original Mongolian word kürügän, "son-in-law".[9][10] Various Persian sources use a byname, Tīmūr-e Lang which translates to "Timur the Lame", as he was lame after sustaining an injury to the leg in battle. In the West, he is commonly known as Tamerlane, which derives from his Persian by name.
Timur was born in Transoxiana, near Kesh (an area now better known as Shahrisabz, 'the green city) some 50 miles south of Samarkand in modern Uzbekistan. His father Taraghay was the head of the Barlas, a nomadic tribe in the steppes of Central Asia. They were remnants of the original Mongol hordes of Genghis Khan, many of whom had embraced Turkic or Iranian languages and customs.
Timur was a Muslim. The spurious genealogy on his tombstone tracing his descent back to Ali, as well as the presence of Shiites in his army, led some observers and scholars to call him a Shiite. However, his official religious counselor was the Hanafite scholar 'Abdu 'l-Jabbar Khwarazmi. He also constructed one of his finest buildings at the tomb of Ahmed Yesevi, an influential Turkic Sufi saint who was spreading Sunni Islam among the nomads.
In about 1360 Timur gained prominence as a military leader whose troops were mostly Turkic tribesmen of the region.. He took part in campaigns in Transoxania with the Khan of Chagatai, a fellow descendant of Genghis Khan. His career for the next ten or eleven years may be thus briefly summarized from the Memoirs. Allying himself both in cause and by family connection with Kurgan, the dethroner and destroyer of Volga Bulgaria, he was to invade Khorasan at the head of a thousand horsemen. This was the second military expedition which he led, and its success led to further operations, among them the subjection of Khwarizm and Urganj.
After the murder of Kurgan the disputes which arose among the many claimants to sovereign power were halted by the invasion of the energetic Jagataite Tughlugh Timur of Kashgar, another descendant of Genghis Khan. Timur was dispatched on a mission to the invader's camp, the result of which was his own appointment to the head of his own tribe, the Barlas, in place of its former leader, Hajji Beg.
The exigencies of Timur's quasi-sovereign position compelled him to have recourse to his formidable patron, whose reappearance on the banks of the Syr Darya created a consternation not easily allayed. The Barlas were taken from Timur and entrusted to a son of Tughluk, along with the rest of Mawarannahr; but he was defeated in battle by the bold warrior he had replaced at the head of a numerically far inferior force.
Rise to power
Tughlugh's death facilitated the work of recon quest, and a few years of perseverance and energy sufficed for its accomplishment, as well as for the addition of a vast extent of territory. It was in this period that Timur reduced the Jagatai khans to the position of figureheads, who were deferred to in theory but in reality ignored, while Timur ruled in their name. During this period Timur and his brother-in-law Husayn, at first fellow fugitives and wanderers in joint adventures full of interest and romance, became rivals and antagonists. At the close of 1369 Husayn was assassinated and Timur, having been formally proclaimed sovereign at Balkh, mounted the throne at Samarkand, the capital of his dominions. This event was recorded by Marlowe in his famous work Tamburlaine the Great:
Then shall my native city, Samarcanda... Be famous through the furthest continents,
For there my palace-royal shall be placed, Whose shining turrets shall dismay the heavens,
And cast the fame of Ilion's tower to hell. A legendary account of Timur's rise to leadership, recorded among the Tatar descendants of the Qıpchaq Khanate in Tobol, goes as follows:
One day Aksak Temür spoke thusly:
"Khan Züdei (in China) rules over the city. We now number fifty to sixty men, so let us elect a leader." So they drove a stake into the ground and said: "We shall run thither and he who among us is the first to reach the stake, may he become our leader". So they ran and Aksak Timur (since he was lame) lagged behind, but before the others reached the stake he threw his cap onto it. Those who arrived first said: "We are the leaders". (But) Aksak Timur said: "My head came in first, I am the leader". In the meanwhile an old man arrived and said: "The leadership should belong to Aksak Timur; your feet have arrived but, before then, his head reached the goal". So they made Aksak Timur their prince.
It is notable that Timur never claimed for himself the title of khan, styling himself Amir and acting in the name of the Chagatai ruler of Transoxania. Timur was a military genius but sometimes lacking in political sense. He tended not to leave a government apparatus behind in lands he conquered, and was often faced with the need to conquer such lands again after inevitable rebellions.
Timur spent the next 35 years in various wars and expeditions. He not only consolidated his rule at home by the subjugation of his foes, but sought extension of territory by encroachments upon the lands of foreign potentates. His conquests to the west and northwest led him among the Mongols of the Caspian Sea and to the banks of the Ural and the Volga. Conquests in the south and south-West encompassed almost every province in Persia, including Baghdad, Karbala and Northern Iraq.
One of the most formidable of his opponents was another Mongol ruler, descendant of Genghis Khan Tokhtamysh who, after having been a refugee at the court, became ruler both of the eastern Kipchak and the Golden Horde and quarrelled with him over the possession of Khwarizm and Azerbaijan. Timur supported him against Russians and Tokhtamysh, with armed support by Timur, invaded Russia and in 1382 captured Moscow. After the death of Abu Sa'id, ruler of the Ilkhanid Dynasty, in 1335, there was a power vacuum in the Persian Empire. In 1383 Timur started the military conquest of Persia. He captured Herat, Khorasan and all eastern Persia by 1385 and captured almost all of Persia by 1387. These conquests were characterised by exceptional brutality. For example, when Isfahan surrendered to Timur in 1387, he initially treated it with relative mercy as he commonly did with cities that surrendered without resistance. However, after the city revolted against Timur's punitive taxes by killing the tax collectors and some of Timur's soldiers, Timur ordered the complete massacre of the city, killing a reported 70,000 citizens. An eye-witness counted more than 28 towers, each constructed of about 1,500 heads.
In the meantime, Tokhtamysh, now khan of the Golden Horde, turned against his patron and invaded Azerbaijan in 1385. It was not until 1395, in the battle of Kur River, that Tokhtamysh's power was finally broken after a titanic struggle between the two monarchs. In this war, Timur first led an army of over 100,000 men north for more than 700 miles into the uninhabited steppe, then west about 1000 miles, advancing in a front more than 10 miles wide. The Timurid army almost starved, and Timur organized a great hunt where the army encircled vast areas of steppe to get food. Tokhtamysh's army finally was cornered against the Volga River in the Orenburg region and destroyed. During this march, Timur's army got far enough north to be in a region of very long summer days, causing complaints by his Muslim soldiers about keeping a long schedule of prayers in such northern regions. Timur led a second campaign against Tokhtamysh via an easier route through the Caucasus. Timur then destroyed Sarai and Astrakhan, and wrecked the Golden Horde's economy based on Silk Road trade.
Indian Campaign
Timur began a trek starting in 1398 to invade the reigning Sultan Nasir-u Din Mehmud of the Tughlaq Dynasty in the north Indian city of Delhi.[17] His campaign was politically pretexted that the Muslim Delhi Sultanate was too tolerant toward its Hindu subjects, but that could not mask the real reason being to amass the wealth of the Delhi Sultanate.
Timur crossed the Indus River at Attock (now Pakistan) on September 24, 1398, but Timur's invasion did not go unopposed and he did meet some resistance during his march to Delhi, by the Governor of Meerut. Timur was able to continue his relentless approach to Delhi, arriving in 1398 to combat the armies of Sultan Mehmud, already weakened by an internal battle for ascension within the royal family.
The Sultan's army was easily defeated on December 17, 1398. On this day the army of Sultan Mahmud Khan had prepared 120 war elephants armoured with chain mail. He had put poison on the tusks, which put fright into the Tartar lines. Timur took action and the Tartars dug out a trench in front of their positions. Timur then took his camels and placed all the wood and hay he could on their backs. When the war elephants charged he lit the camels on fire and then prodded them with iron sticks. They charged at the elephants howling in pain. Timur had understood that elephants were easy creatures of panic. Faced with the strange spectre of the burning camels flying straight at them with flames leaping from their backs, the elephants turned around and stampeded back toward their own lines. Timur entered Delhi and the city was sacked, destroyed, and left in ruins. Before the battle for Delhi, Timur executed a large number of captives, mostly Hindus.
Timur himself recorded the invasions in his memoirs, collectively known as Tuzk-e-Taimuri.In them, he vividly described the massacre at Delhi:
Contribution of ART
Timur became widely known as a patron to the arts. Much of the architecture he commissioned still stands in Samarkand, now in present-day Uzbekistan. He was known to bring the most talented artisans from the lands he conquered back to Samarkand, and is credited with often giving them wide latitude of artistic freedom to express themselves.
According to legend, Omar Aqta, Timur's court calligrapher, transcribed the Qur'an using letters so small that the entire text of the book fit on a signet ring. Omar also is said to have created a Qur'an so large that a wheelbarrow was required to transport it. Folios of what is probably this larger Qur'an have been found, written in gold lettering on huge pages.
Timur was also said to have created Tamerlane Chess, a variant of shatranj (also known as medieval chess) played on a larger board with several additional pieces and an original method of pawn promotion. These pieces included the camel, siege-weapon, giraffe, and several others as well as boasting a complicated system involving the ability to exchange pawns for certain pieces should they reach the other side of the board.
Timur's mandating of Kurash wrestling for his soldiers ensured for it a lasting and legendary legacy. Kurash is now a popular international sport and part of the Asian Game.
LEGACY
Timur's legacy is a mixed one. While Central Asia blossomed under his reign, other places such as Baghdad, Damascus, Delhi and other Arab, Persian, Indian and Turkic cities were sacked and destroyed. Thus, while Timur still retains a positive image in Central Asia, he is vilified by many in Arab, Persian and Indian societies.
Timur's military talents were unique. He used propaganda in what is now called information warfare as part of his tactics. His campaigns were preceded by the deployment of spies whose tasks included collecting information and spreading horrifying reports about the cruelty, size, and might of Timur’s armies. Such disinformation eventually weakened the morale of threatened populations and caused panic among enemy forces. He planned all his campaigns years in advance, including planting barley for horse feed two-years ahead of his campaigns. Whilst Timur’s uncharacteristic (for the time) concern for his troops inspired fierce loyalty he did not pay them. Their only incentives were from looting captured territory — a bounty that included horses, wives, precious metals and stones; in other words whatever they, or their newly indentured slaves, could carry away from the conquered lands.
Timur's short-lived empire also melded the Turko-Persian tradition in Transoxiania, and in most of the territories which he incorporated into his fiefdom, Persian became the primary language of administration and literary culture (diwan), regardless of ethnicity.[30] In addition, during his reign, some contributions to Turkic literature were penned, with Turkic cultural influence expanding and flourishing as a result. A literary form of Chagatai Turkic came into use alongside Persian as both a cultural and an official language.
Timur became a popular figure in Europe for centuries after his death, not in the least because of his victory over the Ottoman Sultan and the humiliations to which he is said to have subjected his prisoner Bayezid.
Timur was officially recognised as a national hero of newly independent Uzbekistan. His monument in Tashkent takes the place where Marx's statue once stood.
Exhumation
Timur's body was exhumed from his tomb in 1941 by the Soviet anthropologist Mikhail M. Gerasimov. From his bones it was clear that Timur was a tall and broad chested man with strong cheek bones. Gerasimov also found that Timur's facial characteristics conformed to partial Mongoloid features, which he believed, in some part, supported Timur's notion that he was descended from Genghis Khan. Gerasimov was able to reconstruct the likeness of Timur from his skull.
His height was 5 foot 8 inches (1.73 meters), tall for his era. He also confirmed Timur's lameness due to a hip injury. Timur was re-buried with full Islamic ritual in November 1942 just before the Soviet victory at the Battle of Stalingrad (ref Marozzi 2004)
Timur's tomb is protected by a slab of jade in which are carved the words in Arabic: "When I rise, the World will Tremble". It is said that when Gerasimov exhumed the body, an additional inscription inside the casket was found reading "Whosoever opens my tomb shall unleash an invader more terrible than I." In any case, two days after Gerasimov had begun the exhumation, Nazi Germany launched Operation Barbarossa, its invasion of the U.S.S.R.
14.09.2009. Time: 22.00
DISABILITIES & BLUE BADGE ABUSE IN THE UK

The Disability Discrimination Act
• access to goods, facilities and services, including larger private clubs and land-based transport services
• buying or renting land or property, including making it easier for disabled people to rent property and for tenants to make disability-related adaptations
• functions of public bodies, for example issuing of licences
The Disability Rights Commission (DRC) closed on 28 September 2007, but the archived website is still available and has plenty of information, including a brief overview with the key points of the Act.

There is a huge access problem in the UK such as:
1) Shops;
2) Cinema & Theatre;
3) Train & Underground;
4) Street and Pavement;
5) Access to educational establishment;
6) Public buses and taxes;
7) Sport facilities
8) Disability has been one of the unfortunate plagues hampering normal life and dreams of African People.
The disabled have been denied attention for so long and need to be treated with absolute LOVE AND PASSION as love-making in a warm KENTE. Africans love and cherish kente, and acquire it with passion because it is symbolises our cultural heritage.
Our love for the disabled strengthens them and makes them fight on, to make a living for themselves. It simply takes them off our streets and enables them regain their confidence knowing that they are not facing life all alone but with our care and support.

Nature also suffers disabilities
Disable Blue Badges have been abused by none disable people. It is been sold for a couple of thousands among the none disable people.


The tallest people on earth have so many problems:
1) They cannot freely travel because people keep looking at them;
2) They cannot buy clothes because there in any size to fit them.
3) Bed is another problem, they need 3 meter bed.
4) Transport is another problem, they must have their own vehicles.
5) Low ceiling room is a big problem, because they have to bend all the time.
6) Home must be adapted for their need which cost huge.
7) Marrying is another problem. Because girls look at their abnormal size.
7) Finding job is another big problem.
Date:20.09.2009. Time: 22.00
WHAT IS E- COLI

E. coli are not always confined to the intestine, and their ability to survive for brief periods outside the body makes them an ideal indicator organism to test environmental samples for faecal contamination. The bacteria can also be grown easily and its genetics are comparatively simple and easily-manipulated or duplicated through a process of mutagenic, making it one of the best-studied prokaryotic model organisms, and an important species in biotechnology and microbiology. E.Coli has been present for years in South Tipperary drinking water, especially in Burncourt water scheme.
E. coli was discovered by German paediatrician and bacteriologist Theodor Escherichia in 1885, and is now classified as part of the Enterobacteriaceae family of gamma-proteobacteria.
New strains of E. coli evolve through the natural biological process of mutation, and some strains develop traits that can be harmful to a host animal. Although virulent strains typically cause no more than a bout of diarrheal in healthy adult humans, particularly virulent strains, such as O157:H7 or O111:B4, can cause serious illness or death in the elderly, the very young or the immunocompromised.
BIOLOGY
E. coli is Gram-negative, facultative anaerobic and non-sporulating. Cells are typically rod-shaped and are about 2 micrometres (μm) long and 0.5 μm in diameter, with a cell volume of 0.6 - 0.7 μm it can live on a wide variety of substrates. E. coli uses mixed-acid fermentation in anaerobic conditions, producing lactate, succinate, ethanol, acetate and carbon dioxide. Since many pathways in mixed-acid fermentation produce hydrogen gas, these pathways require the levels of hydrogen to be low, as is the case when E. coli lives together with hydrogen-consuming organisms such as methanogens or sulphate-reducing bacteria.
Optimal growth of E. coli occurs at 37°C but some laboratory strains can multiply at temperatures of up to 49°C. Growth can be driven by aerobic or anaerobic respiration, using a large variety of redox pairs, including the oxidation of pyruvic acid, formic acid, hydrogen and amino acids, and the reduction of substrates such as oxygen, nitrate, dimethyl sulfoxide and trimethylamine N-oxide.
Strains that possess flagella can swim and are motile. The flagella have a peritrichous arrangement.
GASTROINTESTINAL
Intestinal mucosa-associated E. coli are observed in increased numbers in the inflammatory bowel diseases, Crohn's disease and ulcerative colitis.[20] Invasive strains of E. coli exist in high numbers in the inflamed tissue, and the number of bacteria in the inflamed regions correlates to the severity of the bowel inflammation.
Epidemiology of gastrointestinal infection
Shiga toxin-producing E. coli (STEC), specifically serotype O157:H7, have also been transmitted by flies, as well as direct contact with farm animals, petting zoo animals, and airborne particles found in animal-rearing environments.
In stool samples microscopy will show Gram negative rods, with no particular cell arrangement. Then, either MacConkey agar or EMB agar (or both) are inoculated with the stool. On MacConkey agar, deep red colonies are produced as the organism is lactose positive, and fermentation of this sugar will cause the medium's pH to drop, leading to darkening of the medium. Growth on Levine EMB agar produces black colonies with greenish-black metallic sheen. This is diagnostic of E. coli. The organism is also lysine positive, and grows on TSI slant with a (A/A/g+/H2S-) profile. Also, IMViC is ++-- for E. coli; as it's indol positive (red ring) and methyl red positive (bright red), but VP negative (no change-colourless) and citrate negative (no change-green colour). Tests for toxin production can use mammalian cells in tissue culture, which are rapidly killed by shiga toxin. Although sensitive and very specific, this method is slow and expensive.
The Council of State and Territorial Epidemiologists recommend that clinical laboratories screen at least all bloody stools for this pathogen. The American Gastroenterological Association Foundation (AGAF) recommended in July 1994 that all stool specimens should be routinely tested for E. coli O157:H7. It is recommended that the clinician check with their state health department or the Centres for Disease Control and Prevention to determine which specimens should be tested and whether the results are reportable.
Other methods for detecting E. coli O157 in stool include ELISA tests, colony immunoblots, direct immunofluorescence microscopy of filters, as well as immunocapture techniques using magnetic beads these assays are designed as screening tool to allow rapid testing for the presence of E. coli O157 without prior culturing of the stool specimen.
ANTIBIOTIC TREATMENT
Antibiotic resistance is a growing problem. Some of this is due to overuse of antibiotics in humans, but some of it is probably due to the use of antibiotics as growth promoters in food of animals.[45] A study published in the journal Science in August 2007 found that the rate of adaptative mutations in E. coli is "on the order of 10–5 per genome per generation, which is 1,000 times as high as previous estimates," a finding which may have significance for the study and management of bacterial antibiotic resistance.
To prevent problem
1) Wash hands with soap if one is touched animal.
2) See your GP if any abnormal fever or stomach upset occur.